英语邮件信件里“最后”应该用final还是last还是其他的？

比如Have a nice day Enjoy the holiday To your success Keep up the good workBest wishes All the best Good luck! Take care and see you soonWith best wishes for the ……这三句前面要是加个“最后”的话应该用什么词，要加逗号还是直接放进句子里一起用？